![]() ![]() ![]() Materials & Methods This descriptive study was conducted during 2008 to 2010 in three periods and 90 samples from 30 sites were collected, totally. Regarding to the application of leeches in medicine and their fast extinction, this study was performed in aquatic habitats of Kashan aimed to determine the distribution of leeches and to provide information about their regional and habitat characteristics. Naragh Suburb, Center of Iran A Natural Habitat of Hirudo medicinalisĭirectory of Open Access Journals (Sweden)įull Text Available Aims A very common species of leeches has been named as Hirudo medicinalis. They are also an appropriate training module for neuroscience or physiology teaching laboratories. ![]() These leech preparations are still widely used to address basic electrical properties of neural networks, behavior, synaptogenesis, and development. Then we demonstrate how to prepare a patch of innervated skin to be used for mapping sensory or motor fields. Next we show how individual cells of known function can be removed from the ganglion to be cultured in a Petri dish, and how to record from those neurons in culture. We demonstrate how to dissect the leech for recording intracellularly from identified neural circuits in the ganglion. The goal of this report is to consolidate experimental techniques from the leech system into a single article that will be of use to physiologists with expertise in other nervous system preparations, or to biology students with little or no electrophysiology experience. The freshwater leech, Hirudo medicinalis, is a versatile model organism that has been used to address scientific questions in the fields of neurophysiology, neuroethology, and developmental biology. Titlow, Josh Majeed, Zana R Nicholls, John G Cooper, Robin L Intracellular recording, sensory field mapping, and culturing identified neurons in the leech, Hirudo medicinalis. ![]()
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